ebioscience

时间:2024-08-15 01:18:44编辑:小松

journal of biosciences审稿快么

Journal of biosciences
影响因子: 1.759
I S S N: 0250-5991
出 版 社: Springer India, in co-publication with Indian Academy of Sciences
出 版 地: New Delhi
出版国家: India
刊  期: 季刊
创刊时间: 1979
语  种: 英文
审稿周期: 较慢,6-12周
5年影响因子: 2.218
研究领域: 生物化学、生物
官方链接: ias.ac.in/jbiosci/
投稿须知: link.springer.com/journal/12038
期刊介绍:
Published by the Indian Academy of Sciences, the Journal of Biosciences covers all areas of Biology and is India’s premier journal within its scope. It is indexed in Current Contents and other standard Biological and Medical databases.
The Journal of Biosciences began in 1934 as the Proceedings of the Indian Academy of Sciences (Section B). In 1978 it split into Proceedings-Animal Sciences, Proceedings-Plant Sciences and Proceedings-Experimental Biology. Proceedings-Experimental Biology was renamed Journal of Biosciences in 1979; in 1991, it merged with Proceedings-Animal Sciences and Proceedings-Plant Sciences.
Articles span a broad range of relevant topics, including, for example, protein classification by surface comparisons; the contribution of bioinformatics to genome and genomics research, and mathematical modelling of spatio-temporal interactions within the cell.


流式细胞术实验步骤是什么?

流式细胞术实验:1、制备细胞悬液,计数2、分装,按照每个EP(1.5ml)管1-2*10 6个细胞分装,3500rpm,4度离心。3、用100ulPBS重悬,加入10ul大鼠血清封闭,4度避光30min。4、加入相应的荧光标记的抗体,混匀,避光,4度孵育30min。5、加入1mlPBS洗两遍。6、用200ul PBS重悬,经纱布过滤转至流式管,上级检测。细胞或者大小接近的颗粒物质都可以检测。样本通过鞘液的输送,成单个队列依次通过激光束。样本事先可以根据检测需要被荧光标记,通过激光束的时候就会得到荧光信号,被记录下来。可以同时标记不同的荧光标记做不同的检查。优点就是可以在短时间内检测大量的样本(每秒几千个到几万个细胞)。主要应用于各种生物医学研究和临床诊断。国际上通用的白细胞和淋巴瘤的诊断标准就是靠流式细胞仪的分型来确定的。以上内容参考:百度百科-流式细胞术

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